|Place of Origin:||China, Shanghai|
|Certification:||NQA ISO 9001:2015|
|Minimum Order Quantity:||100ug|
|Packaging Details:||ice packaging, carton|
|Delivery Time:||7 weekdays|
|Supply Ability:||1000g per week|
|Product:||Sequencing Grade Trypsin||Cat. No.:||SRT0201|
|CAS:||9002-07-7||Specific Activity:||>4500 USP Units/mg Pro|
|EC:||220.127.116.11||Contaminant Activity:||No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.|
sequencing grade modified trypsin,
ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein
Sequencing Grade Trypsin
YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥4500 USP units/mg pro.|
|SDS-PAGE||Single major band|
|Molecular Weight(SDS-PAGE)||24.0±2.4 kDa|
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.
2. When using this product, thaw it at room temperature, and mix gently before use.
3. No activity lost when freeze-thaw 5 cycles.
1. The solution should be stored under -70℃, It is stable within 24 months.
2. Above 95% activity remained after 5 times repeated freezing and thawing.
3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.
The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.
Contact Person: Miss Eland