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ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein

ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein

  • ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein
  • ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein
  • ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein
  • ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein
ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRT0201
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 98$/100ug
Packaging Details: ice packaging, carton
Delivery Time: 7 weekdays
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Sequencing Grade Trypsin Cat. No.: SRT0201
CAS: 9002-07-7 Specific Activity: >4500 USP Units/mg Pro
EC: 3.4.21.4 Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.
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sequencing grade modified trypsin

,

trypsin endopeptidase

ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein

 

Sequencing Grade Trypsin

 

DESCRIPTION

YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

MAIN FEATURES

Source Recombinant E. coli
Appearance White or off white, or yellowish powder
Specific activity ≥4500 USP units/mg pro.
SDS-PAGE Single major band
Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
Purity(HPLC) ≥95%

 

Unit Definition

One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

Application

Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

 

Reconmend Usage

1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.

2. When using this product, thaw it at room temperature, and mix gently before use.

3. No activity lost when freeze-thaw 5 cycles.

 

Storage Instructions

1. The solution should be stored under -70℃, It is stable within 24 months.

2. Above 95% activity remained after 5 times repeated freezing and thawing.

3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.

 

PRODUCT USAGE

The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.

 

ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein 0

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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