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Human Trypsin, Recombinant Trypsin Solution, No EDTA

Human Trypsin, Recombinant Trypsin Solution, No EDTA

  • Human Trypsin, Recombinant Trypsin Solution, No EDTA
  • Human Trypsin, Recombinant Trypsin Solution, No EDTA
  • Human Trypsin, Recombinant Trypsin Solution, No EDTA
  • Human Trypsin, Recombinant Trypsin Solution, No EDTA
Human Trypsin, Recombinant Trypsin Solution, No EDTA
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RTS04
Payment & Shipping Terms:
Minimum Order Quantity: 100ml
Packaging Details: 100ml, 500ml; ice packing, carton
Payment Terms: T/T in advance
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Detailed Product Description
EDTA: Without EDTA Formula With: Human Trypsin
Source: E.coli Name: Recombinant Trypsin Solution
Band: Yaxin Feature: Animal Component-free
High Light:

lyophilized powder

,

trypsin cell culture

Human Trypsin, Recombinant Trypsin Solution, No EDTA

 

Recombinant Trypsin Solution

 

DESCRIPTION

Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography.

YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is 7.0-11.0.

 

MAIN FEATURES

Source Recombinant E. coli
Appearance White or off white, or yellowish powder
Specific activity ≥3800 USP units/mg pro.
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 24.0±2.4 kDa

UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

RECOMMEND USAGE

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

 

FEATURES AND BENEFITS

Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination

Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.

Recombinant trypsin

Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.

High purity

Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.

High safety

The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.

Convenient

Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.

Good work without EDTA

No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.

 

 

 

Human Trypsin, Recombinant Trypsin Solution, No EDTA 0

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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