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Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine

Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine

  • Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine
  • Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine
  • Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine
  • Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine
Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RHT03
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 89$/10mg
Packaging Details: 1mg, 10mg, 100mg, 1g; ice packaging, carton
Delivery Time: 5 days
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Recombinant Human Trypsin 2 Manufacturer: YaxinBio
Specific Activity: ≥2500 USP U/mg Pro. Purified by : HPLC
Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant. Purity: > 99%
High Light:

stem cell culture

,

trypsinized cells

Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine

 

Human  Trypsin

 

Description

 

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.

Recombinant Human Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

 

Advantages

 

Animal origin free:The use of recombinant Human Trypsin eliminates the risk of virus presence, and of any other potential adventitious agents found in animal pancreas-derived trypsin. YaxinBio Recombinant Human Trypsin belongs to the AOF level 3.

Recombinant human trypsin:The amino acid sequence is the same as the Human Trypsin 2.

Stable:A sterile recombinant human trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant human trypsin provides increased specificity and eliminates contaminating activities found in lower purity enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

3) Purity is more than 95% by HPLC.

 

Unit Definition

 

One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

RECOMMEND USAGE

Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

 

 

Human Trypsin, Cleave C-terminal Peptide Bond of Lysine and Arginine 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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