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Enzyme Markers Monoclonal and Polyclonal Antibody Purification

Enzyme Markers Monoclonal and Polyclonal Antibody Purification

  • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
  • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
  • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
  • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
  • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
Enzyme Markers Monoclonal and Polyclonal Antibody Purification
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RSPA05
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 79$/10mg
Packaging Details: 10mg, 100mg, 1g; ice packaging, carton
Delivery Time: 5-8 weekdays
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Substrate: Porous Polystyrene-divinylbenzene Particle Size: 40 Um
Ligand: Recombinant Protein A Recommend Flow Rate: 300-700 Cm/h
DBC: At Least 300 Mg IgG/mL Media At 500 Cm/h Maximum Pressure: 1500 Psi
Brand: YaxinBio
High Light:

protein A column

,

purified protein

Enzyme Markers Monoclonal and Polyclonal Antibody Purification

 

Recombinant Protein A

 

Catalog Number:RSPA05

CAS:91932-65-9

Storage temperature: 2-8℃

Source:Protein A mutant of Staphylococcus aureus , expressed in E. coli

 

1.DESCRIPTION

Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies from different subtypes of mammals or genetically engineered recombinant proteins containing antibody Fc fragments. alkali protein A is our company independent research and development production, with independent intellectual property rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more. The purification process does not use antibody column affinity chromatography to avoid the possibility of incorporation of unrelated IgG in the product.

 

2.MAIN FEATURES

Appearance White or off white powder
Solubility Clear liquid
Bioburden ≤ 1 cfu/ mg
SDS-PAGE Single major band
SDS-PAGE (MW) 25000±1000 Daltons
UV Spectrum E275/250 ≥ 1.2
E0.1% at 275nm = 0.17-0.23
Purity (HPLC) ≥ 95%

 

3.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.

After dissolved with pure water, it should be stored under -20℃.

Stability of transport: The product is stable by blue ice insulation transport.

 

 

4.APPLICATION:

The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.

 

5.Product Specification

Test/Method                    

Specification

Bioburden

No organisms detected.

12% SDS-PAGE

Single major band

Coomassie Stain

25kD

UV Spectrum

E275/250 ≥1.2,

E0.1% at 275nm=0.17-0.23 

UV spectrometry

No DNA or RNA detected

Triton Content

No Triton contaminant.

Format

Lyophilized

pH stable range

pH 1-1

 

 

 

 

 

 

 

 

 

 

 

 

 

6.Storage Instructions

Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.

 

7.Usage

For Research or Manufacturing Purpose Only. Not for Human.

 

 

8.RELATED PRODUCTS

Recombinant carboxypeptidase B

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase

Enzyme Markers Monoclonal and Polyclonal Antibody Purification 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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