|Place of Origin:||Shanghai|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||10mg, 100mg, 1g; ice packaging, carton|
|Payment Terms:||T/T in advance|
|Supply Ability:||1000g per week|
|Ligand:||Recombinant Protein A||Recommend Flow Rate:||300-700 Cm/h|
|PH:||PH 2-10||Temperature Range:||2-8℃|
protein A column,
ELISA or Immunohistochemical Tests Fluorescent Molecules, CAS 91932-65-9
Recombinant protein A
Storage temperature: 2-8℃
Source:Protein A mutant of Staphylococcus aureus , expressed in E. coli
Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies from different subtypes of mammals or genetically engineered recombinant proteins containing antibody Fc fragments. alkali protein A is our company independent research and development production, with independent intellectual property rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more. The purification process does not use antibody column affinity chromatography to avoid the possibility of incorporation of unrelated IgG in the product.
|Appearance||White or off white powder|
|Bioburden||≤ 1 cfu/ mg|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||25000±1000 Daltons|
|UV Spectrum||E275/250 ≥ 1.2|
|E0.1% at 275nm = 0.17-0.23|
|Purity (HPLC)||≥ 95%|
3.STABILITY OF STORAGE AND TRANSPORT
(1)Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.After dissolved with pure water, it should be stored under -20℃.
(2)Stability of transport: The product is stable by blue ice insulation transport.
For Research or Manufacturing Purpose Only. Not for Human.
The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.
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Contact Person: Miss Eland