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EDTA, Proteomics Grade Trypsin, TPCK Trypsin, Recombinant Trypsin

EDTA, Proteomics Grade Trypsin, TPCK Trypsin, Recombinant Trypsin

  • EDTA, Proteomics Grade Trypsin, TPCK Trypsin, Recombinant Trypsin
  • EDTA, Proteomics Grade Trypsin, TPCK Trypsin, Recombinant Trypsin
  • EDTA, Proteomics Grade Trypsin, TPCK Trypsin, Recombinant Trypsin
  • EDTA, Proteomics Grade Trypsin, TPCK Trypsin, Recombinant Trypsin
EDTA, Proteomics Grade Trypsin, TPCK Trypsin, Recombinant Trypsin
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RPT0201
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 65$/10mg
Packaging Details: ice packaging, carton
Delivery Time: 8 days
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Manufacturer: YaxinBio Packaging: 10mg,100mg,1g Or Bulk
Source: E. Coli Physical Form: White Lyophilized Powder
Related Product: Recombinant Human Trypsin
High Light:

cell culture trypsin

,

trypsin enzyme

EDTA, Proteomics Grade Trypsin, TPCK Trypsin, Recombinant Trypsin

 

Recombinant Trypsin

 

Description

 

Enzyme Commission (EC) Number: 3.4.21.4
Molecular Weight: 23.3 kDa (bovine & porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5(bovine)

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end

of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7.0 - 9.0. The enzyme

is inhibited by serine protease inhibitors, e.g. PMSF, aprotinin, soybean trypsin inhibitor and by

metal chelating agents, e.g., EDTA.

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

by high pressure liquid chromatography. There are no any other contaminating enzyme activities.

No protease inhibitors such as PMSF are contained in the preparation.

 

Advantages

 

Animal origin free:The use of recombinant porcine trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.

Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

   2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

 

Main Features

 

Purified by HPLC
Specific activity ≥3800 USP units/mg pro
Purity(RP-HPLC) NLT 70% β-trysin, NMT 20% α-trypsin
Contaminant activity No chymotrypsin, carboxypeptidase A, and other protease contaminant.

 

 

 

 

 

 

Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute

in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

Recommend Usage

 

To prepare 1-10mg/ml recombinant trypsin with 1mM HCl. The ratio to aimed protein is 1:50 to

1:1000 (w/w), the optimum pH is pH7-10.

 

Storage And Stability

 

Recommend recombinant trypsin lyophilized powder stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

EDTA, Proteomics Grade Trypsin, TPCK Trypsin, Recombinant Trypsin 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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