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Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli.

Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli.

  • Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli.
  • Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli.
  • Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli.
  • Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli.
Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli.
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RPT0201
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: $65/10mg
Packaging Details: 1mg, 10mg, 100mg, 1g; ice packaging, carton
Delivery Time: 7 Days
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Recombinant Porcine Trypsin Packaging: 10mg,100mg,1g Or Bulk
Physical Form: White Or White-like Lyophilized Powder Additives: Carbohydrates
Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant. Related Product: Recombinant Aprotinin
Expressed: E.coli.
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lyophilized powder

,

trypsin cell culture

Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli.
 

Recombinant Trypsin (solution)

 

Description

 

Recombinant trypsin solution is a defined, animal component-free solution developed for efficient dissociation of adherent cell types from culture surfaces and tissues. This solution is optimized for sensitive cells, such as primary human mesenchymal stem cells (hMSC).

The recombinant trypsin solution is a pure enzyme solution, which maximizes the yield of functionally viable cells from culture vessels, while preventing toxicity induced by other contaminating proteases. This solution does not contain undesirable proteins such as carboxypeptidase A and chymotrypsin, which are commonly found in porcine or bovine-derived trypsin solutions. In addition, recombinant trypsin solution eliminates the risk of viruses and other potential adventitious agents that can be carried in animal-derived components.

 

Advantages

 

Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.

Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

 

Main Features

 

1.Defined, animal component-free

2.Ready-to-use solution

3.Pure, recombinant trypsin - no contaminating proteases

4.Optimized for sensitive cells, such as hMSCs

5.Compatible with cells cultured in both serum-free and serum-containing media

Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

Recommend Usage

 

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-10.0.

 

Storage Instructions

 

Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

 

Related Product

 

Recombinant Carboxypeptidase B

Recombinant Chymotrypsin

Recombinant Enterokinase

Cell Dissociation Solution, Recombinant Trypsin, Expressed in E.coli. 0

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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