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Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin

Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin

  • Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin
  • Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin
  • Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin
  • Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin
Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: $198/100ug
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000 per week
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Detailed Product Description
Product: Sequencing Grade Carboxypeptidase B Manufacturer: YaxinBio
Source: E. Coli Specific Activity: NLT 200 Units/mg Pro
Contaminant Activity: No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Sequencing Grade Chymotrypsin
Impact: Produce Insulin
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trypsin endopeptidase

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carboxypeptidase enzyme

Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin

 

Recombinant Carboxypeptidase B

 

Description

 

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

 

MAIN FEATURES

 

Source Recombinant E. coli
Appearance White or off white, or yellowish powder
Specific activity ≥170 units/mg pro.
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 33.8±3.4 kDa

UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.

 

RECOMMEND USAGE

 

Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.

 

Recommend Usage

 

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

 

Storage Instructions

 

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.

Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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