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Carboxypeptidase B, For Cell Culture and Protein Digestion

Carboxypeptidase B, For Cell Culture and Protein Digestion

  • Carboxypeptidase B, For Cell Culture and Protein Digestion
  • Carboxypeptidase B, For Cell Culture and Protein Digestion
  • Carboxypeptidase B, For Cell Culture and Protein Digestion
  • Carboxypeptidase B, For Cell Culture and Protein Digestion
Carboxypeptidase B, For Cell Culture and Protein Digestion
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 150$/10mg
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Recombinant Carboxypeptidase B Cat. No.: RCPB01
Specific activity: ≥170 U/mg Pro Additives: Tris Salts, NaCl Salts And Carbohydrates
Protein content: 35% ~ 60% Purity: > 95%
High Light:

trypsin endopeptidase

,

carboxypeptidase enzyme

Carboxypeptidase B, For Cell Culture and Protein Digestion

 

Recombinant Carboxypeptidase B

 

DESCRIPTION

 

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

 

Main Features

 

Source

E. Coli

Purified by

HPLC

Specific activity

 ≥170 units/mg pro.

Purity

Single main band on SDS-PAGE

M.W.

33.8kD

Contaminant activity

Less than 10 ppm of recombinant trypsin.

 

 

 

 

 

 

 

Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.

 

Recommend Usage

 

To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

 

STABILITY OF STORAGE AND TRANSPORT

 

Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.

It is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

Storage Instructions

 

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

 

Carboxypeptidase B, For Cell Culture and Protein Digestion 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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