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Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli

Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli

  • Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli
  • Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli
  • Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli
  • Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli
Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: REK08
Payment & Shipping Terms:
Minimum Order Quantity: 100U
Price: 65$/100u
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 10000ku per week
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Detailed Product Description
EK Content: 1-2U Temperature: 25℃
Storage: Store At -20°C After Delivery. Specific Activity: One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein.
Fusion Protein Concentration: 0.1-1mg/ml (total Protein Content: 0.5-1.0mg) Cutting Condition: Given An Example: 25mM Tris-HCl 8.0
Source: Recombinant E. Coli
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bovine enterokinase

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enterokinase enzyme

Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli

 

Recombinant Enterokinase

 

Catalog Number:REK08

CAS:9017-74-8
EC:3.4.21.9

Source: bovine enterokinase, expressed in E. Coli

 

 

Description

 

YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.

 

 

Product Overview

 

Recombinant Bovine Enterokinase produced in E.coli is a single glycosylated polypeptide chain containing 235 amino acids and having a molecular mass of approximately28 kDa.

 

Advantages

 

1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K)

2) No any other contaminated proteases, no non-specific cutting sites.

 

Recommend Usage Condition

 

Cutting condition given an example: 25mM Tris-HCl 8.0
Fusion protein concentration 0.1-1mg/ml (total protein content: 0.5-1.0mg)
EK content 1-2U
Temperature 25℃
Time overnight or 16h-24h for digestion

 

 

 

 

 

 

 

Common Components Influence the Action of Enterokinase

 

>200mM imidazole or >200mM NaCl or >5%glycerin, the reaction may be effected.The following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.

Bovine Light Chain Enterokinase, Recombinant Enterokinase, Expressed in E.coli 0

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Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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