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Animal Origin Free, Recombinant Human Trypsin, for Treating Cell

Animal Origin Free, Recombinant Human Trypsin, for Treating Cell

  • Animal Origin Free, Recombinant Human Trypsin, for Treating Cell
  • Animal Origin Free, Recombinant Human Trypsin, for Treating Cell
  • Animal Origin Free, Recombinant Human Trypsin, for Treating Cell
  • Animal Origin Free, Recombinant Human Trypsin, for Treating Cell
Animal Origin Free, Recombinant Human Trypsin, for Treating Cell
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RHT03
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 89$/10mg
Packaging Details: 1mg, 10mg, 100mg, 1g; ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Source: E. Coli Cat. No.: RHT03
Manufacturer: YaxinBio Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant.
Related Product: Recombinant Porcine Trypsin Packaging: 10mg,100mg Or Bulk
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trypsin digestion

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stem cell culture

Animal Origin Free, Recombinant Human Trypsin, for Treating Cell

 

Recombinant Human Trypsin

 

CAS: 9002-07-7
EC: 3.4.21.4

Source: human trypsin, expressed in E. Coli.

 

Description

 

1.Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues.pH7.0-9.0 is the optimum pH. Serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA can inhibite the trypsin enzyme.

 

2.Recombinant human trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

 

MAIN FEATURES

Source Recombinant E. coli
Appearance White or off white powder
Specific activity ≥2500 USP units/mg pro.
Protein Content ≥ 90%
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
Purity(HPLC) ≥ 95%

UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

RECOMMEND USAGE

Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

 

Storage Instructions

Recombinant human trypsin lyophilized powder should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

Animal Origin Free, Recombinant Human Trypsin, for Treating Cell 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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