|Place of Origin:||China, Shanghai|
|Certification:||NQA ISO 9001:2015|
|Minimum Order Quantity:||1g|
|Packaging Details:||ice packaging, carton|
|Supply Ability:||1000g per week|
|Purified By:||HPLC||Format:||White Lyophilized Powder|
|Specific Activity:||≥3800 USP U/mg Pro||Purity(RP-HPLC):||NLT 70% β-trysin, NMT 20% α-trypsin|
Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified
by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
|BAEE Units/ml enzyme =||(ΔA253nm/min Test - ΔA253nm/min Blank)(df)|
df = Dilution factor
0.001 = The change in A253nm/minute per unit of Trypsin at pH 7.6 at 25 °C in a 3.2 ml reaction
mix 0.20 = Volume (in milliliters) of enzyme used
|Units/mg solid=||units/ml enzyme|
|mg solid/ml enzyme|
1.This assay procedure is not to be used to assay immobilized trypsins
2.For the USP/NF procedure refer to the USP monograph
3.This procedure is for informational purposes. For a current copy of Sigma’s quality control procedure contact our Technical Service Department.
1 TAME µM Unit = 0.27 BAEE µM Units
1 BAEE µM Unit = 3.64 TAME Units
1 TAME µM Unit = 55 BAEE A253 Units
1 BAEE A253 Unit = 0.018 TAME µM Unit
1 TAME µM Unit = 180 TAME A247 Units
1 TAME A247 Unit = 0.33 BAEE Units
1 USP Unit = 3.0 BAEE Units
1 NF Unit = 1.1 USP Units
Animal origin free: The use of recombinant Porcine Trypsin eliminates the risk of virus presence,
and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant
Porcine Trypsin belongs to the AOF level 3.
Stablility: A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable
within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and
above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland