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Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro

Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro

  • Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
  • Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
  • Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
  • Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
  • Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RPT0201
Payment & Shipping Terms:
Minimum Order Quantity: 1g
Price: 450$/g
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Purified By: HPLC Format: White Lyophilized Powder
Specific Activity: ≥3800 USP U/mg Pro Purity(RP-HPLC): NLT 70% β-trysin, NMT 20% α-trypsin
Source: E. Coli
High Light:

porcine trypsin

,

trypsin enzyme

Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro

 

Recombinant Trypsin

 

Description

 

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

Calculation

BAEE Units/ml enzyme = (ΔA253nm/min Test - ΔA253nm/min Blank)(df)
(0.001) (0.20)

 

 

 

df = Dilution factor
0.001 = The change in A253nm/minute per unit of Trypsin at pH 7.6 at 25 °C in a 3.2 ml reaction

mix 0.20 = Volume (in milliliters) of enzyme used

Units/mg solid= units/ml enzyme
mg solid/ml enzyme

 

 

 

Notes
1.This assay procedure is not to be used to assay immobilized trypsins
2.For the USP/NF procedure refer to the USP monograph
3.This procedure is for informational purposes. For a current copy of Sigma’s quality control procedure contact our Technical Service Department.

Unit Conversions
1 TAME µM Unit = 0.27 BAEE µM Units
1 BAEE µM Unit = 3.64 TAME Units
1 TAME µM Unit = 55 BAEE A253 Units
1 BAEE A253 Unit = 0.018 TAME µM Unit
1 TAME µM Unit = 180 TAME A247 Units
1 TAME A247 Unit = 0.33 BAEE Units
1 USP Unit = 3.0 BAEE Units
1 NF Unit = 1.1 USP Units

 

Advantages

 

Animal origin free: The use of recombinant Porcine Trypsin eliminates the risk of virus presence,

and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant

Porcine Trypsin belongs to the AOF level 3.

Stablility: A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

 

Recommend Usage

 

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.

 

Storage Instructions

 

Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable

within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and

above 90% activity remained after 10 times repeated freezing and thawing.

Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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