|Place of Origin:||Shanghai|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||1mg, 10mg, 100mg, 1g; ice packaging, carton|
|Payment Terms:||T/T in advance|
|Supply Ability:||1000g per week|
|Product:||Sequencing Grade Carboxypeptidase B||Source:||Recombinant E. Coli|
|Additives:||Tris Salts, NaCl Salts And Carbohydrates||Packaging:||10mg,100mg,1g,bulk|
Animal Origin Free, Carboxypeptidase B, For Protein Structure and Sequence Analysis
Recombinant Carboxypeptidase B
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Purity by HPLC
≥ 200 units/mg pro
No chymotrypsin, carboxypeptidase A, or other proteases contaminant.
Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.
Antibody quality control.
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.
Contact Person: Miss Eland