Home ProductsRecombinant Carboxypeptidase B

Animal Origin Free, Carboxypeptidase B, For Arg-C and lys-C, Sequencing Grade

Animal Origin Free, Carboxypeptidase B, For Arg-C and lys-C, Sequencing Grade

  • Animal Origin Free, Carboxypeptidase B, For Arg-C and lys-C, Sequencing Grade
  • Animal Origin Free, Carboxypeptidase B, For Arg-C and lys-C, Sequencing Grade
  • Animal Origin Free, Carboxypeptidase B, For Arg-C and lys-C, Sequencing Grade
  • Animal Origin Free, Carboxypeptidase B, For Arg-C and lys-C, Sequencing Grade
Animal Origin Free, Carboxypeptidase B, For Arg-C and lys-C, Sequencing Grade
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 198$/100ug
Packaging Details: 1mg, 10mg, 100mg, 1g; ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1,000g per week
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Detailed Product Description
Product: Sequencing Grade Carboxypeptidase B Manufacturer: YaxinBio
Source: E. Coli Specific Activity: NLT 200 Units/mg Pro
Contaminant Activity: No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Sequencing Grade Chymotrypsin
High Light:

sequencing grade modified trypsin

,

trypsin endopeptidase

Animal Origin Free, Carboxypeptidase B, For Arg-C and lys-C, Sequencing Grade

 

Recombinant Carboxypeptidase B

 

Catalog Number:RCPB01

CAS: 9025-24-5
EC: 3.4.17.2

Storage temperature: 2-8℃

Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli

Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B

 

1.DESCRIPTION

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

 

2.MAIN FEATURES

Source Recombinant E. coli
Appearance White or off white, or yellowish powder
Specific activity ≥170 units/mg pro.
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 33.8±3.4 kDa

UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.

 

3.RECOMMEND USAGE

Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.

 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.

It is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

5.PRODUCT ADVANTAGE

① Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not

used in the production process.

② Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and

the product quality is stable.

③ High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host

protein residues is less than the limits of biological products.

④ Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.

 

⑤ Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory

requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

⑥ Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

 

6.PRODUCT USAGE

The production of recombinant insulin and its analogues;

the quality test of recombinant antibodies;

the determination of C-terminal amino acids of proteins;

the production of other recombinant polypeptides;

and the enzymatic synthesis of certain specific compounds.

 

7.RELATED PRODUCTS

Recombinant trypsin

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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